AP Biology Unit 6: Gene Expression and Regulation — Mind Map
Covers DNA replication, transcription, translation, the genetic code, gene regulation (operons in prokaryotes, transcription factors in eukaryotes), mutations and their consequences, and core biotechnology techniques (PCR, gel electrophoresis, recombinant DNA, CRISPR).
Gene Expression and Regulation
Genetic Material
DNA Structure
Double Helix
Antiparallel Strands
Deoxyribose Sugar
Bases: A, T, C, G
RNA Structure
Single Stranded
Ribose Sugar
Bases: A, U, C, G
DNA Replication
Semiconservative Model
5' to 3' Direction
Enzymes
Helicase: Unwinds DNA
Topoisomerase: Relieves strain
DNA Polymerase: Adds nucleotides
Ligase: Joins fragments
Primase: Adds RNA primers
Strands
Leading: Continuous
Lagging: Okazaki fragments
Gene Expression Process
Transcription
RNA Polymerase
Promoter Region
mRNA Synthesis
RNA Processing
Intron Excision
Exon Splicing
5' GTP Cap
Poly-A Tail
Translation
Ribosomes
tRNA and Anticodons
Codons
Polypeptide Chain
Regulation of Expression
Prokaryotic (Operons)
Inducible: Lac Operon
Repressible: Trp Operon
Operator and Repressor
Eukaryotic
Chromatin Accessibility
Histone Acetylation
DNA Methylation
Transcription Factors
miRNA Degradation
Mutations
Types
Silent: No change
Missense: Different amino acid
Nonsense: Stop codon
Frameshift: Insertion/Deletion
Functional Effects
Loss-of-function
Gain-of-function
Null alleles
Biotechnology Tools
PCR: DNA Amplification
Gel Electrophoresis: Separation
DNA Sequencing
Bacterial Transformation
AcornPrep